Anti-SIRT6 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-SIRT6 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0740

The Silent Information Regulator (Sir2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of this family is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging. SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway. The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation, and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H2O2. In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres, and gaps. SirT6 may regulate the BER pathway by deacetylating DNA Polβ or other core components of the pathway.

UniProt ID:

Q8N6T7

Overview

Reactivity: Human, mouse, rat, bovine, chicken, Drosophilia melanogaster, Zebrafish

Tested applications: Western Blotting 1:1000

Immunofluorescence 1:500

Immunohistochemistry (Paraffin) 1:500

Immunoprecipitation(IP) 1;100
ChIP, CUT&RUN and CUT&Tag

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total SirT6 protein. This antibody does not cross-react with other sirtuin proteins.

Properties

Immunogen: Synthetic peptide:GLPEIFDPPEELERK conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 19-33 of Human SIRT6

Clonality: Monoclonal, clone number: 9F21

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: SIRT6 Antibody - Detection of SIRT6 in HeLa whole cell lysate.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: SIRT6 Antibody - SIRT6 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Anti-SIRT6 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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