Anti-STAT6 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Overview

Reactivity

Mouse, Rat, Human

Tested applications

WB	: 1/500-1/2000.	molecular weight: 94kDa.
IHC	: 1/100~1/200
IF/ICC	: 1/50~1/200
CoIP	: 1/50
ChIP	: 10-20µL per reaction
CUT&RUN	: 2-10µL per reaction
CUT&Tag	: 2-10µL per reaction

 
There are feedbacks from end-users that can be done:ChIP, CUT&RUN and CUT&Tag
Optimal dilutions/concentrations should be determined by the end user.

Specificity

Recognizes endogenous levels of total Stat6 protein.

Properties

Immunogen

Recombinant protein encompassing a sequence within the center region of human STAT6. The exact sequence is proprietary. Swiss-Prot Acc: P42226

Clonality

Monoclonal, clone number: 4R2

Isotype

Rabbit IgG

Storage instruction

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C

Applications

WB Image

Western blot –Stat6 antibody (AT0606) at 1/1000 dilution
 
Sample: in  HELA cell lysate and 293 cell lysate;
Observed band size: 94 kDa
 
Recommended secondary antibody: Goat anti rabbit IgG-HRP (Engibody, Catalog number:AT0097)
Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

IHC Image

Immunohistochemistry-Paraffin: Stat6 Antibody
 
Analysis of Paraffin-embedded mouse kidney tissue using anti-Stat6 antibody. Counter stained with hematoxylin.

ICC/IF Image

Immunocytochemistry/Immunofluorescence: Stat6 Antibody
 
Staining Stat6 in hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

IP/CoIP Image

Immunoprecipitation: Stat6 Antibody
 
Detection of human Stat6 in HeLa nuclear extracts, input, Stat6 IP and IgG IP.
 
Stat6 immunoprecipitated with this antibody(1:50) and WB detected with this antibody(1:1000).