Anti-Smad2 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-Smad2 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0368

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

Swiss-Prot Acc:Q15796

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blot: 1:1000

Immunohistochemistry: 1:500

Immunoprecipitation: 1:100
ChIP, CUT&RUN and CUT&Tag

Specificity: Detects endogenous levels of total Smad2 protein. This antibody does not cross-react with Smad3.

Properties

Immunogen: Produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse Smad2 protein.

Clonality: Monoclonal, clone number: 7A1

Isotype: Rabbit IgG

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C

Applications

WB Image: Western Blot: Smad2 Antibody - Analysis of Smad2 on different lysates using anti-Smad2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: PC12

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Smad2 Antibody - Staining Smad2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Anti-Smad2 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Related Products

Reviews