Anti-Smad4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-Smad4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0647

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the recepter-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Briefly, activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved SSXS motif at the carboxy-terminus of the proteins. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad, Smad4, and together the complex moves to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blotting 1:2000

Immunoprecipitation 1:100

Immunohistochemistry (Paraffin) 1:500

Immunofluorescence (Immunocytochemistry) 1:300

Flow Cytometry 1:200

ChIP, CUT&RUN and CUT&Tag

Specificity: This antibody detects endogenous levels of total Smad4 protein.

Properties

Immunogen: Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Smad4.

Clonality: Monoclonal, clone number: 3DA7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot:

Smad4 Antibody - Analysis of Smad4 on PC-12 cells lysates using anti-Smad4 antibody at 1/1,000 dilution.

IHC Image: Immunohistochemistry-Paraffin:

Smad4 Antibody - Analysis of paraffin-embedded mouse colon tissue using anti-Smad4 antibody. Counter stained with hematoxylin.

ICC/IF Image: Immunocytochemistry/Immunofluorescence:

Smad4 Antibody - Staining Smad4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Anti-Smad4 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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