Anti-Smad5 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-Smad5 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0648

Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis. BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors. They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad9 (Smad8) at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes.

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blotting 1:2000

Immunoprecipitation 1:100

Immunohistochemistry (Paraffin) 1:500

Immunofluorescence (Immunocytochemistry) 1:300

Flow Cytometry 1:200

ChIP, CUT&RUN and CUT&Tag

Specificity: This antibody detects endogenous levels of total Smad5 protein.

Properties

Immunogen: Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human SMAD5.

Clonality: Monoclonal, clone number: 7GA3

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: Smad5 Antibody - Analysis of Smad5 on PC-3M cell lysates using anti-Smad5 antibody at 1/1,000 dilution.

IHC Image: Immunohistochemistry-Paraffin: Smad5 Antibody - Analysis of paraffin-embedded mouse testis tissue using anti-Smad5 antibody. Counter stained with hematoxylin.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: Smad5 Antibody - Staining Smad5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Anti-Smad5 Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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