Anti-Smoothened Rabbit mAb

Overview

Description

Rabbit monoclonal antibody to Smoothened

Reactivity

Mouse, Human

Tested applications

WB: 1:1000-1:5000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).

IHC-P: 1:50-1:200

ICC/IF: 1:50-1:200

Properties

Immunogen

Recombinant Human Smoothened homolog protein

Clonality

Monoclonal, clone number: 23B7

Isotype

Rabbit IgG

Form

Liquid

Preservative: 0.03% Proclin 300

Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage instruction

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Database links

• SwissProt: Q99835 Human
• SwissProt: P56726 Mouse

Applications

WB Image

Positive WB detected in: A549 whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, U251 whole cell lysate, Hela whole cell lysate, Mouse Brain tissue lysate, Rat Brain tissue lysate

 

All lanes : Anti-Smoothened antibody (AT0402) at 1:1000

Predicted band size : 86 kDa
Observed band size : 86 kDa

ICC/IF Image

Immunofluorescence staining of A549 cell with AT0402 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Application Figure 3

Immunofluorescence staining of SY5Y cell with AT0402 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).