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    Anti-Sodium Potassium ATPase (ATP1A1) rabbit mAb - Cell Membrane Loading Control

    Catalog number :AT0813
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    Background: 
    The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic α subunit and a β subunit. Several phosphorylation sites have been identified for the α1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase, Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA. All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule. Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the α subunit. Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors.
     
    Overview
    Reactivity
    Mouse, Rat, Human
    Tested applications
    Western Blotting: 1/1000-1/5000
     
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user. 

    Product Picture
    Anti-Sodium Potassium ATPase (ATP1A1) rabbit mAb - Cell Membrane Loading Control
    Specificity
    This antibody recognizes endogenous levels of total Na,K-ATPase α1 protein. Based on sequence homology, the antibody is likely to cross-react with α2 and α3 isoforms. A doublet may form if samples are boiled.
    Properties
    Immunogen
    a synthetic peptide corresponding to residues near the amino terminus of human Na,K-ATPase α1 subunit. Antibodies are purified using peptide affinity chromatography.
    Clonality
    Monoclonal, clone number: 6P12
    Isotype
    Rabbit IgG
    Form
    Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol,0.02% Sodium Azide
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.
    Host
    Rabbit
    Applications
    WB Image

    Western Blot: Sodium Potassium ATPase Alpha 1 Antibody - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.

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