V5-Alpaca-HRP Anti-V5 tag Alpaca Nanobody(VHH) Conjugated HRP

Product

V5-Alpaca-HRP Anti-V5 tag Alpaca Nanobody(VHH) Conjugated HRP

Catalog number :AT8323

V5-Alpaca-HRP Anti-V5 tag Alpaca Nanobody(VHH) Conjugated HRP is designed for detecting V5 tag (GKPIPNPLLGLDST) fusion proteins specifically. V5-Alpaca-HRP Anti-V5 tag Alpaca Nanobody(VHH) Conjugated HRP is recombinant alpaca nanobody conjugated HRP. It detects the V5 tag (GKPIPNPLLGLDST) selectively, no reactivity with other proteins.

The V5 tag is a 14 amino acid peptide derived from a small epitope on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, V5 tag is unlikely to affect the tagged protein’s biochemical properties. V5 tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Overview

Description: V5-Alpaca-HRP Anti-V5 tag Alpaca Nanobody(VHH) Conjugated HRP(Horseradish Peroxidase)

Reactivity: Detect transfected V5 tag (GKPIPNPLLGLDST) fusion proteins expressed in E. coli, yeast, insect cell, mammalian cell, or in vitro transcription/translation systems. All systems that express V5 tag (GKPIPNPLLGLDST) fusion protein are suitable.

Tested applications: WB: 1/2000 - 1/10000,
ELISA: 1/2000 - 1/10000

There is no need to use secondary antibody

Product Picture

Specificity: V5 tag sequence(GKPIPNPLLGLDST)

Properties

Immunogen: V5 tag fusion protein

Clonality: Alpaca monoclonal nanobody(VHH,single domain antibody), without Fc fusion

Isotype: VHH domain of alpaca IgG2b/2c

Form: Liquid

Storage instruction: Store at –20 °C, (Avoid freeze / thaw cycles)

Conjugation: conjugated HRP(Horseradish Peroxidase)

Host: Alpaca pacous

Storage buffer: In 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol, and 0.02% ProClin 300 as preservative. No BSA.

Purity: Recombinant expression in yeast and affinity purified

Applications

Highlights: Benefits Of Alpaca Nanobody

No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments

Highly specific binding

Low background

High affinity

High stability

Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells

Animal-free production, recombinant expression in yeast and affinity purified

V5-Alpaca-HRP Anti-V5 tag Alpaca Nanobody(VHH) Conjugated HRP

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