Anti-alpha smooth muscle Actin Rabbit mAb
Catalog number :AT0666
Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells.
- Overview
- Reactivity
- Mouse, Rat, Human
- Tested applications
- Western Blotting 1:1000-1:10000Immunohistochemistry (Paraffin) 1:200-1:500Flow Cytometry 1:50Optimal dilutions/concentrations should be determined by the end user.
- Specificity
- This antibody recognizes endogenous levels of total α-smooth muscle protein.
- Properties
- Immunogen
- Synthetic peptide corresponding to Human alpha smooth muscle Actin. This antibody was raised against a synthetic peptide corresponding to N-terminus of actin from human smooth muscle.
- Clonality
- Monoclonal, clone number: 5DS7
- Isotype
- Rabbit IgG
- Form
- Liquid, 50 μl,1mg/ml, PBS (pH 7.2) and 50% Glycerol,0.02% Sodium Azide
- Storage instruction
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.
- Host
- Rabbit
- Applications
- WB Image
Western Blot: alpha-Smooth Muscle Actin Antibody - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha smooth muscle Actin antibody diluted at a dilution of 1:2000.
- IHC Image
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody - Paraffin-embedded mouse colon. alpha smooth muscle Actin antibody dilution: 1:500.
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