Anti-beta Catenin Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-beta Catenin Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0622

beta -Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blot 1:500-1:3000

Immunohistochemistry-Frozen 1:100-1:1000

Immunohistochemistry-Paraffin 1:100-1:1000
ChIP, CUT&RUN and CUT&Tag

Specificity: This Antibody detects endogenous levels total of β-catenin. This antibody does cross-react with endogenous levels of γ-catenin.

Properties

Immunogen: Synthetic peptide: PGDSNQLAWFDTDL

conjugated to KLH, corresponding to amino acids 768-781 of Human or mouse beta Catenin.

Clonality: Monoclonal, clone number: 5FA7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C

Host: Rabbit

Applications

WB Image: Western Blot: beta-Catenin Antibody - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

IHC Image: Immunohistochemistry-Paraffin: beta-Catenin Antibody - Staining of human cervix, uterine shows moderate membranous positivity in squamous epithelial cells.

ICC/IF Image: Immunofluorescence: beta-Catenin Antibody - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

Anti-beta Catenin Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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