CoIP, ChIP, CUT&RUN, CUT&Tag Expert! My Basket (...)
Worldwide delivery!
Having the Best Alpaca Nanobody Products!
Home | About us | Contact us | How to order |
    My Base Info
    Shopping Basket
    My Orders
    My Shipping Info
    Modify Password
    Log Out

    Anti-beta III Tubulin Rabbit mAb - Neuronal Marker

    Catalog number :AT0673
    loading...
    The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins.
     
    Beta 3-tubulin (TUBB3) is one of six β-tubulin isoforms and is expressed highly during fetal and postnatal development (axon guidance and maturation). Its expression levels decrease in the adult central nervous system (CNS) but remain high in the peripheral nervous system (PNS). Microtubules enriched in β3-tubulin are more dynamic than those composed of other β-tubulin isoforms. Research studies have shown that mutations in the β3-tubulin gene TUBB3 cause ocular motility defects and other nervous system disorders. Furthermore, β3-tubulin is present in neoplastic but not in normal differentiated glial cells. Thus, β3-tubulin is a great neuronal marker.
     
    Overview
    Reactivity
    Human, Mouse, Rat, Monkey
    Tested applications
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Immunohistochemistry (Paraffin) 1:500
    Optimal dilutions/concentrations should be determined by the end user. 
     
    Specificity
    This antibody detects endogenous levels of total β3-tubulin protein. This antibody does not cross-react with tubulin isoforms expressed in non-neuronal cells.
    Properties
    Immunogen
    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human neuron specific beta III Tubulin.
    Clonality
    Monoclonal, clone number: 8D3
    Isotype
    Rabbit IgG
    Form
    Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol,0.02% Sodium Azide
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.
    Host
    Rabbit
    Applications
    WB Image

    Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue. PVDF membrane was probed with dilution(1:1000) of Anti-Neuron-specific beta ‑III Tubulin Rabbit polyclonal Antibody (Catalog: AT0673) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog: AT0097). A specific band was detected for beta ‑III Tubulin at approximately 55 kDa (as indicated).

    ICC/IF Image

    beta  III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using dilution(1:500) of Anti-Neuron-specific beta  III Tubulin Rabbit polyclonal Antibody (Catalog: AT0673)  and 10 µg/mL anti Nestin Antigen Affinity-purified mouse monoclonal Antibody. Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta III Tubulin using the NorthernLights™ 557-conjugated Anti-rabbit IgG Secondary Antibody (red) and for Nestin using the NorthernLights 493-conjugated Anti-mouse IgG Secondary Antibody (green). Tissue was counterstained with DAPI (blue). 

    Related Products

    Reviews

    loading...
    Content *:
    Customer Review :
    Verification Code * :
    refresh image

    Shipping and Paying Info
    ......
    Order Information
    You can place an order online step by step as blow.

    Step 1. Register for a new account and Log in, Find out your product by searching our website.
    Step 2. Add the Product into your shopping basket, select your settlement currency.
    Step 3. Checkout by Paypal, Pay by credit card or bank transfer for your order.
    Step 4. Ship the goods for you, providing you package tracking numbers. 

    Any questions, please contact us via email: sale@engibodybio.com
    Payments by
    All our products are For Research Use Only. Not for diagnostic or therapeutic usages.