Anti-Caspase 3 Rabbit mAb

Catalog number :AT0769

Background:

The caspase-3 precursor (32kDa) is first cleaved at Asp175-Ser176 to produce the p12 subunit

and the p19 protein. Subsequently, the p19 protein is cleaved at Asp28-Ser29 to generate the

mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and

two p12 subunits.

At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond.

During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the

membrane of the ER in a proteolytic reaction that is distinct from their normal steroldependent

activation. Caspase-3 cleaves and activates SREBPs between the basic helixloop-helix leucine

zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates

caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly

expressed in lung, spleen, heart, liver, kidney and cells of the immune system.

UniProt ID: P42574

Overview

Tested applications: Western Blotting 1:1000

Calculated molecular weight: 32kDa.

Observed molecular weight: 35kDa.

Immunofluorescence 1:200

Immunohistochemistry (Paraffin) 1:200

Immunoprecipitation(IP) 1:50

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects inactive (pro) forms of the enzymes. 35kD

Properties

Immunogen: Recombinant full-length human Caspase-3 protein (pro-form) was used as immunogen.

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Applications

WB Image: Western blot - Caspase 3 antibody (AT0769) at 1/1000 dilution

Observed band size: 35kDa

Recommended secondary antibody: Goat anti rabbit IgG-HRP

(Engibody, Catalog number:AT0097)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent

HRP Substrate (Engibody, Catalog number: IF6747)

ICC/IF Image: Immunofluorescent analysis of A673

cells, using pro Caspase 3 Antibody.

Antibody at 1:100 for 3 hours at room

temperature. Cells were stained using

the ifluor 488-conjugated Anti-Rabbit

IgG Secondary Antibody (Green) and

counterstained with DAPI (blue).