Membrane-based blotting applications that employ enzyme conjugates to generate colorimetric or chemiluminescent signal require the use of an added blocking step to decrease the signal generated by non-specific binding. 5x Blocking Buffer is a caseinbased buffer designed to provide the highest possible signal-to-noise performance, while eliminating the inconvenience associated with the preparation of casein-based blocking buffers. 5x Blocking Buffer functions with both neutral and positively charged membranes and is recommended for both colorimetric and chemiluminescent detection.