Alpaca-CoIP-RAB™ Anti-Rabbit IgG Alpaca Nanobody (HRP) for IP/CoIP

Product

Alpaca-CoIP-RAB™ Anti-Rabbit IgG Alpaca Nanobody (HRP) for IP/CoIP

Catalog number :AT8303

Rabbit has only one IgG subclass. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 kD and the light chain is about 25 kD. The common IgG is monomeric with a molecular weight of approximately 150 kD.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Alpaca immunoglobulin. The size of VHH is extremely small (<15 kDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus, VHH is considered of great value for research, diagnostics and therapeutics.

Alpaca-CoIP-RAB™ Anti-Rabbit IgG Alpaca Nanobody (HRP) for IP/CoIP is designed for detecting NATIVE Fc region of rabbit IgG(H+L) specifically. It is based on monovalent, recombinant single domain antibodies to rabbit IgG(H+L) coupled to HRP. Based on western blot and/or ELISA, it reacts with NATIVE rabbit IgG(H+L) selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG.

Alpaca-CoIP-RAB™ Anti-Rabbit IgG Alpaca Nanobody (HRP) for IP/CoIP is western blotting reagents that enable the trouble-free detection of western blotted target protein bands from upstream IP/CoIP, without interference from denatured IgG (IP primary antibody). This allows to detect the (co-)immunoprecipitated protein without interfering by the IgG heavy (50 kDa) and light chains (25 kDa). In general, this interference tends to originate from traditional secondary antibodies which recognize primary antibodies released with the antigen during the immunoprecipitation procedure or endogenous IgGs from the lysate itself.

Alpaca-CoIP-RAB™ Anti-Rabbit IgG Alpaca Nanobody (HRP) for IP/CoIP only recognize native (non-reduced) primary antibodies (from rabbit) and therefore the bands of heavy and light chains is highly minimized, if the immunoprecipitated antigen-antibody complex is fully reduced and denatured before downstream WB.

Overview

Description: Alpaca-CoIP-RAB™ Anti-Rabbit IgG Alpaca Nanobody (HRP) for IP/CoIP only recognize native (non-reduced) primary antibodies (from rabbit) and therefore the bands of heavy chain (50kD) and light chain (25kD) are eliminated.

Reactivity: Rabbit IgG(H+L), native conformation antibody specific

Tested applications: WB: 1:2000-1:10000

Specificity: No cross-reactivity with mouse, human, cynomolgus, rat, sheep and guinea pig IgG

Properties

Immunogen: Recombinant Rabbit IgG

Isotype: VHH domain of alpaca IgG2b

Form: Liquid

Storage instruction: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light, Stable for 12 months at -20°C

Conjugation: HRP

Host: Alpaca pacous

Storage buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol

Purity: Recombinant Expression in Yeast and Affinity purified

Applications

Highlights: This alpaca nanobody only recognize native (non-reduced) primary antibodies (from rabbit) and therefore the bands of heavy chain (50kD) and light chain (25kD) are eliminated.

Protocols: Benefits Of Alpaca Nanobody

No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments

Highly specific binding

Low background

High affinity

High stability

Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells

Animal-free production, recombinant expression in yeast and affinity purified

Related Products

Alpaca-CoIP-MOU™ Anti-Mouse IgG Alpaca Nanobody (HRP) for IP/CoIP

Catalog number

AT8304

Alpaca-CoIP-RAB™ Anti-Rabbit IgG Alpaca Nanobody (HRP) for IP/CoIP

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