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    home » Primary Antibodies » Epigenetics

    Anti-7-methylguanosine (m7G) Antibody

    Catalog number :AT15004
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    7-Methylguanosine (m7G) is a modified purine nucleoside. It is a methylated version of guanosine and when found in human urine, it may be a biomarker of some types of cancer. In the RNAs, 7-methylguanosine have been used to study and examine the reaction evolving methylguanosine. It also plays a role in mRNA as a blocking group at its 5´-end. The m7G modification actively participates in biological and pathological functions by affecting the metabolism of various RNA molecules, including messenger RNA, ribosomal RNA, microRNA, and transfer RNA. Increasing evidence indicates a critical role for m7G in human disease development, especially cancer, and aberrant m7G levels are closely associated with tumorigenesis and progression via regulation of the expression of multiple oncogenes and tumor suppressor genes.
    Overview
    Description
    Anti-7-methylguanosine (m7G) Mouse Monoclonal Antibody
    Reactivity
    Species independent
    Tested applications
    MeRIP: 5ug-10ug for 1 Reaction
    Dot blot: 1/1000.
     
    Optimal dilutions should be determined by the end user.
    Specificity
    This antibody reacts to 7-methylguanosine (m7G) in RNA. No cross reactivity with non-methylated guanosine in RNA. Also reacts m7G cap.
     
    The 7-methylguanosine (m7G) cap, also known as N7-MeGppp is located on the 5'-terminus of the eukaryotic mRNA and in certain viral RNAs, lncRNA and pri-miRNAs.
    Properties
    Immunogen
    m7G Small Molecule conjugated to BSA.
    Positive Controls
    Dot Blot: HeLa total RNA and mRNA; m7G(cap)-oligo.
    Applications
    Application Figure 1
    dot-blot-anti-m7g-antibody

    Dot blot using in vitro transcribed RNA
     
    Sample: in vitro transcribed RNA from full-length of RN7SK RNA (RefSeq ID: NR_001445)
    Application Figure 2
    Anti-7-methylguanosine (m7G) Antibody

    meRNA immunoprecipitation from in vitro transcribed RNA
     
    Sample: 2 ug of in vitro transcribed RNA from full-length of RN7SK RNA (RefSeq ID: NR_001445)

    Related Products

    Smart-meRIP™ meRIP Kit
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