Smart-meRIP™ meRIP Kit

Overview

Description

methylated RNA Immunoprecipitation kit, meRIP kit

Product Picture

Applications

Highlights

This kit is suitable for methylation analysis of total RNA, mRNA, lncRNA, and circRNA from cells, animal tissues, plant tissues, blood, and embedded tissues.

 

By combining different antibodies, different methylation types can be detected, such as m6A, m5C, and m7G.

 

This kit is suitable for cytoplasmic RNA methylation and nuclear RNA methylation.

 

This kit adopts an optimized elution system with a better signal-to-noise ratio than the MeRIP process using free m6A elution.

 

This kit contains negative control IgG antibodies for easy quality control in IP experiments.

 

This reagent kit is compatible with downstream RT qPCR and specific methylation of whole transcriptome sequencing.

 

This Kit also includes RNA fragment column purification reagents for easy recovery of post IP RNA, without the need to purchase additional RNA purification kits.

Work Flow Image

Results Display

m 6 A induced the upregulation of LINC00958 in BC cells.

 

A MeRIP-Seq analysis revealed a notable m 6 A modification site at the LINC00958 location. B RT-PCR indicated upregulated METTL3 mRNA expression in the BC cell line (MCF-7). C METTL3 knockdown (sh-METTL3) was transfected into MCF-7 cells to silence METTL3 expression. D M 6 A analysis (colorimetric method) presented m 6 A modification enrichment in sh-METTL3 or sh-NC transfection. E MeRIP-qPCR illustrated the m 6 A-modified LINC00958 level after METTL3 silencing or control. F RNA stability analysis demonstrated the LINC00958 level in MCF-7 cells transfected with METTL3 silencing. **p < 0.01 was considered as significant.