Anti-N6-methyladenosine (m6A) Antibody

Overview

Description

Anti-N6-methyladenosine (m6A) Mouse Monoclonal Antibody

Reactivity

Species independent

Tested applications

MeRIP: 5ug-10ug for 1 Reaction

Dot blot: 1/1000.

ICC: 1/200 - 1/500.

IHC: 1/100 - 1/500.

 

Optimal dilutions should be determined by the end user.

Specificity

This antibody reacts to N6-methyladenosine (m6A) in RNA. No cross reactivity with non-methylated adenosine and m1A in RNA.

Properties

Immunogen

m6A Small Molecule conjugated to OVA.

Applications

Application Figure 1

Dot Blot - Anti-N6-methyladenosine (m6A) antibody

 

To demonstrate the specificity of AT15003 against N6-methyladenosine (m6A), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the oligonucleotides were spotted on the membrane. The antibody was diluted 1/400 in TBS-T containing 10 % skimmed milk and 1% BSA. AT15003 shows a high specificity for the oligonucleotide with the N6-methyladenosine modification.

Application Figure 2

MeRIP-qPCR showed that there was m6A modification in the 3-UTR region of SENP1.

PCR products were used for gel electrophoresis for data visualization.