Western Blot is widely used to detect and compare proteins from a complex mixture utilizing antibody detection on a membrane. Chemiluminescence has become an easy and sensitive method of detection compared to other analysis. Because of the nature of chemiluminescence detection, it is possible to reprobe the separated protein mixture on the membrane. Conventionally, Western blots have been stripped using extremely harsh conditions that may alter the antigen for subsequent immunoprobing. CMCTAG's Stripping buffer is a novel formula provides a gentle method of removing primary and secondary antibodies membranes that allows several reprobings on the same membrane

 

CMCTAG provides a gentler and time-saving solution:

Antibody Stripping Buffer is a novel formula that allows several re-probings on the same membrane. It effectively and almost quantitatively removes primary and secondary antibodies from the blots without significantly affecting the immobilized proteins. This is a gentle method that allows several re-probings on the same membrane. It has been optimized for HRP chemiluminescence substrates in western blotting.

 

As a result, recycling of protein blots with Antibody Stripping Buffer(Medium) is very useful and flexible:

1. it is more economical and less time consuming to reuse the same blot than to prepare several blots

2. for multiple detections on the same blot - save time and simplify and improve accuracy of probed blots overlay 

3. to spare protein samples that are available in limited quantities, difficult to obtain or expensive – only one blot!

4. to analyze samples with different antibodies under identical conditions, e.g. subtype or isoform specific antibodies

5. when the blot gives unexpected results, to confirm a result with the same or different antibody

6. when a blot is mistakenly incubated with wrong antibody

7. to optimize antibody concentrations or to detect a new antigen with different antibodies