Bacterial Protein Extration Kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. This kit is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Bacterial Protein Extration Kit is based on organic buffering agents and utilizes a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into this kit. It has been tested for use with several widely used bacterial strains.