DAPI Staining Solution

Overview

Description

DAPI Staining Solution (Ready to Use)

Tested applications

IHC, ICC, IF, TUNEL, and so on.

Properties

Storage instruction

Store at -20° C, Avoid exposure to light; stable for one year from the date of shipment.

Applications

Protocols

1. After fixing the cell or tissue sample, wash it appropriately to remove the fixative. If immunofluorescence staining is required later, immunofluorescence staining should be performed first, and then DAPI staining should be performed according to the subsequent steps. If no other staining is required, perform DAPI staining directly.

2. For adherent cells or tissue sections, add a small amount of DAPI staining solution and cover the sample. For suspended cells, add at least 3 times the volume of staining solution to the sample to be stained and mix well.

3. Leave at room temperature for 3-5 minutes.

4. Discard DAPI staining solution and wash 2-3 times with TBST, PBS, or physiological saline, each time for 3-5 minutes.

5. Observe directly under a fluorescence microscope or under a fluorescence microscope after encapsulation. When cells undergo apoptosis, the nuclei of apoptotic cells can be seen to be densely stained or fragmented with dense staining.