EGFP Protein

Product

EGFP Protein

Catalog number :AT1698

Purified recombinant Enhanced Green Fluorescent Protein (EGFP); fused to 6xHis-tag

Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9 kDa protein (composed of 238 amino acid residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research.

Enhanced GFP (EGFP, composed of 239 amino acid residues) has the following mutations: M1_S2insV/F64L/S65T/H232L, which make GFP show increased fluorescence and fold more efficiently under 37 degrees C, respectively. EGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.

It is reported to be a rapidly-maturing weak homodimer with moderate acid sensitivity.

Overview

Description: Purified recombinant enhanced green fluorescent protein fused to 6xHis-tag, full length protein. Engineered from Aequorea victoria

Tested applications: Western blot: use ~25 ng - 100 ng EGFP per lane

Immunoprecipitation(IP): use ~5 µg EGFP per 25 µL anti-GFP antibody conjugated beads slurry

SDS-PAGE: use ~ 1 µg EGFP per lane

ELISA: coat ~ 1ng - 50 ng per well of a 96-well plate

Sustained release formulations of protein drugs：used as the model protein

Producing antibody by immunizing animals: used as the immunogen

Product Picture 1

Product Picture 2

Properties

Form: Liquid (supplied in PBS, pH 7.4, 0.09% sodium azide as preservative),
1 mg/ml, 0.2µm Sterile Filtered

Purity: >95% by SDS-PAGE and HPLC analysis.
Endotoxin: Less than 1EU/µg of EGFP as determined by LAL method.

Storage instruction: Store at -20°C. Avoid freeze-thaw cycles. Stable for one year.

Database links: GenBank: AAB02572.1
UniProtKB: C5MKY7

3D Structure
PDB: 2Y0G

Host: Recombinant protein expression in Escherichia coli

Structure Image

Applications

Highlights: EGFP was derived from wild type jellyfish (Aequorea Victoria) GFP with the following mutations:

Sequence

MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK LEHHHHHH

Molecular Weight: 28 kDa including the polyhistidine-tag

EGFP Protein

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