Products
Catalog number | AT1698 |
Tested applications |
Western blot: use ~25 ng - 100 ng EGFP per lane
Immunoprecipitation(IP): use ~5 µg EGFP per 25 µL anti-GFP antibody conjugated beads slurry
SDS-PAGE: use ~ 1 µg EGFP per lane
ELISA: coat ~ 1ng - 50 ng per well of a 96-well plate
Sustained release formulations of protein drugs:used as the model protein Producing antibody by immunizing animals: used as the immunogen |
Highlights |
EGFP was derived from wild type jellyfish (Aequorea Victoria) GFP with the following mutations:
Sequence
MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK LEHHHHHH
Molecular Weight: 28 kDa including the polyhistidine-tag
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Catalog number | AT1953 |
Tested applications | Western blot: use ~25 ng - 100 ng GST protein per lane |
Highlights | sequence
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK
WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN
MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD
VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA
WPLQGWQATF GGGDHPPKSD LVPRGSPGIH RD
Molecular Weight
27.0 kDa
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Catalog number | AT1697 |
Description | Recombinant Multi-Tag Positive Control Protein |
Tested applications | Western blot |