Goat Anti-Rabbit IgG(H+L) (FITC)

Overview

Description

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC)

Reactivity

Rabbit

Tested applications

FC (Flow Cytometry), IF(Immunofluorescent)
FC: The amount of the reagent is suggested to be used ≤ 1 μg/106 cells .
IF: 1/100 ~1/2000
Since applications vary, the appropriate dilutions must be used at an assay dependent concentration.

Product Picture

Properties

Immunogen

Rabbit IgG whole molecule

Clonality

Polyclonal

Isotype

Goat Polyclonal IgG

Form

Liquid

Storage instruction

Store at -20°C. and protected from prolonged exposure to light.

Conjugation

FITC. Ex: 493nm, Em: 528nm

Applications

ICC/IF Image

Immunofluorescence - Goat Anti-Rabbit IgG H&L (FITC) (AT0118)

ICC/IF image of AT0118 stained MCF-7 cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (Anti-MUC1 rabbit monoclonal antibody) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (FITC) (AT0118) used at 1:500 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.