HA-Catch-Aga™ Anti-HA tag Alpaca nanobody Conjugated Agarose Beads

Overview

Description

HA-Catch-Aga™ Anti-HA tag nanobody (from alpaca VHH, Single Domain antibody) conjugated Agarose Beads

Reactivity

This product is useful tool for IP/CoIP/ChIP of HA-tagged proteins, and it recognizes HA-tags placed at N-terminal, C-terminal, or internal site of the fusion protein.

Tested applications

Immunoprecipitation (IP), 

Co-Immunoprecipitation (Co-IP), 

Chromatin Immunoprecipitation (ChIP) 

RNA Binding Protein Immunoprecipitation (RIP)

Enzyme assays

Mass spectrometry

Affinity purification

 

In IP, 25-50 µL of beads suspension for ~500-1000 µL of crude total cell lysate solution.

Optimal dilutions/concentrations should be determined by the end user.

Specificity

This antibody captures HA-tagged proteins exogenously expressed in cells or E. coli. 

Properties

Immunogen

This nanobody is produced from alpaca with a HA tagged protein.

Form

50% beads slurry

Agarose beads Conjugated anti-HA nanobody, the product is supplied in 10 mM PBS, pH 7.4, and 0.02% (w/v) sodium azide as preservative. 

Storage instruction

Stored at 4 °C. Do not freeze

Host

this antibody is nanobody, it is from aplaca, its advantages are as below:

 

• Fast, reliable & efficient one-step immunoprecipitation

 

• Ready-to-use, high stability, high affinity

 

• No heavy & light antibody chains interference in downstream WB

 

• Stable under harsh washing conditions

 

• Suitable for downstream mass spec, CoIP, ChIP, RIP, protien purification and so on

 

• Works in samples from: mammals, plants, bacteria, yeast, insects etc.

Structure Image

Beads Diameter

~ 90 µm

Binding Capacity

~1mg HA tagged protein /ml beads suspension

Applications

Application Image

Highlights