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    Products

    Catalog number AT1762
    DescriptionFlag-Catch-Aga™ Anti-Flag tag nanobody (from alpaca VHH, Single Domain antibody) conjugated Agarose Beads

    Advantage:
    This is the only anti-flag alpaca nanobody conjugated beads in the world, not Fab conjugated beads from mice or rabbits. This anti-flag alpaca nanobody is better than Fab due to its higher stability and affinity. More importantly, downstream WB will not be interfered with antibody light and heavy chains.
     
    On the contrary, mouse Fab will still be bound by downstream secondary antibodies, resulting in interference with light and heavy chains.
    ReactivityThis product is useful tool for IP/CoIP/ChIP of Flag-tagged proteins, and it recognizes Flag-tags placed at N-terminal, C-terminal, or internal site of the fusion protein.
    Catalog number AT1765
    Reactivity
    exogenously overexpressed GFP proteins in cells or E. coli. 
    Species independent
    ImmunogenRecombinant A. victoria GFP protein
    Catalog number AT1763
    DescriptionHA-Catch-Aga™ Anti-HA tag nanobody (from alpaca VHH, Single Domain antibody) conjugated Agarose Beads
    ReactivityThis product is useful tool for IP/CoIP/ChIP of HA-tagged proteins, and it recognizes HA-tags placed at N-terminal, C-terminal, or internal site of the fusion protein.
    Catalog number AT1795
    FormAgarose beads Conjugated anti- mCherry Nanobody, the product is supplied as a 50% suspension in 10 mM phosphate buffered saline (PBS), pH 7.4, and 0.02% (w/v) sodium azide as preservative.
    SpecificityThis antibody detects mCherry -tagged proteins exogenously expressed in cells or E. coli. This antibody does not detect GFP-tagged proteins. 
    Please note that the mCherry tags add approximately 28 kDa to the molecular weight of the fusion protein.
    Catalog number AT1764
    DescriptionMyc-Catch-Aga™ Anti-Myc tag nanobody (from alpaca VHH, Single Domain antibody) conjugated Agarose Beads
    ReactivityThis product is useful tool for IP/CoIP/ChIP of Myc-tagged proteins, and it recognizes Myc-tags placed at N-terminal, C-terminal, or internal site of the fusion protein.
    Catalog number AT1769
    Tested applications
    Immunoprecipitation (IP), 
    Co-Immunoprecipitation (Co-IP), 
    Chromatin Immunoprecipitation (ChIP) 
    RNA Binding Protein Immunoprecipitation (RIP)
    Enzyme assays
    Mass spectrometry
    Affinity purification
     
    In IP, 25 µL of beads suspension for ~500 µL of crude total cell lysate solution.
    Optimal dilutions/concentrations should be determined by the end user.
    Reactivity
    exogenously overexpressed RFP fusion proteins in cells or E. coli.
    Species independent
    Payments by
    All our products are For Research Use Only. Not for diagnostic or therapeutic usages.