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    Anti-MBP tag Mouse mAb

    Catalog number :AT0030
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    Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP-tag antibody is suitable for detecting fusion proteins that contain a MBP-tag.
    Overview
    Description
    Mouse monoclonal antibody to MBP-Tag [7B10] - Epitope Tag Antibody
    Reactivity
    Species independent
    Tested applications
    WB    : 1/1000-1/5000.
    Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
    Product Picture
    Anti-MBP tag Mouse mAb
    Specificity
    This antibody detects recombinant proteins fused to maltose binding protein (MBP).
    Properties
    Immunogen
    Recombinant full-length MBP protein.
    Clonality
    7B10
    Isotype
    Mouse IgG1
    Form
    Liquid, 100 μg at 1mg/ml

    Mouse monoclonal antibody in 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 1 mg/mL (0.1% w/v) BSA (IgG free, protease free) as a stabilizer and 0.02% (w/v) sodium azide as preservative.
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C
    Applications
    WB Image

    Western blot analysis of MBP-tag fusion protein with anti MBP-tag mouse mAb [7B10] diluted at 1: 2000.

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