Myc peptide for Elution

Product

Myc peptide for Elution

Catalog number :P5203

Myc Peptide is a synthetic peptide with a molecular weight of 1203.3. The amino acid sequence is: N-Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-C, corresponding to amino acids 410-419 of the C-terminal of human c-myc.

The peptide is useful for competing out anti-Myc tag antibody binding to Myc-tagged fusion proteins in immunoassays.

Overview

Tested applications: For use in competitive elution of Myc tagged fusion proteins from the anti-Myc monoclonal antibody (Cat no: AT0023) in solution or bound to agarose beads on the anti-Myc agarose affinity gel (Cat no: AT0080).

Properties

Sequence	GLU-GLN-LYS-LEU-ILE-SER-GLU-GLU-ASP-LEU
Sequence name	EQKLISEEDL
Molecular Formula	C₅₁H₈₆N₁₂O₂₁
Molecular Weight	1203.3
Purity	> 95%
Storage	Store lyophilized reagent at –20 °C. For extended storage after reconstitution, store at –20 °C in working aliquots. Avoid repeated freeze-thaw.

Form	The product is supplied as lyophilized powder.
Working Concentration	Recommended working concentration is 100 µg/ml-1mg/ml for elute Myc tagged fusion proteins from the anti-Myc agarose affinity gel(Cat no: AT0080). Note: In order to obtain best results in different techniques we recommend determining optimal working dilution by titration test.
Preparation of stock solution	To prepare a stock solution, dissolve in water to a final concentration of 5 mg/ml. Aliquot and store at –20 °C.