QuickGel™ PAGE 10% Gel Preparation Kit

Overview

Description

QuickGel™ PAGE 10% Gel Preparation Kit (5% Stacking Gel,10% Resolving Gel)

Tested applications

• Native PAGE or SDS-PAGE

• Western blotting

Properties

Components

Sufficient For: 120 mini gels

 

• 2xResolving gel(10%): 250ml;   

• 2xResolving gel buffer: 250ml

• 2xStacking gel(5%): 80ml;      

• 2xStacking gel buffer: 80ml;

• Enhanced APS solution: 8ml (APS solution should be stored at -20° C.)

• Preparation Cup

Storage instruction

Store at 4° C; stable for one year from the date of shipment.

APS solution should be stored at -20° C for long term.

Applications

Highlights

Safer

Avoid contact with highly toxic acrylamide powder, and do not use the odor TEMED to protect the safety of laboratory staffs. 
 

Fast and simple

No need to weigh, no need to dilute, just take and use, just add a proper amount of APS to directly pour gel.
 

The bands are more cohesive

With patented formula, the bands are more cohesive and non-dispersive, and the resolution of protein migration is increased.
 

Higher stability

The pre prepared gel solution avoids potential weighing errors.
 

More efficient

The well-proportioned liquid saves the weighing step.

Work Flow Figure 1

Work Flow Figure 2

Protocols

PAGE Gels Preparation Procedure:

1. Clean surfaces of gel plates first. Assemble glass plates sandwich.

 

Attention: Make sure to bring solutions out of refrigerator back to room temperature (20-25°C) before use. 

APS solution can be stored at 4° C after the first use, or aliquot at 1 ml and store at -20°C, avoid repeated freeze/thaw cycles.

 

2. Prepare Resolving gel mixture.

According to gel dimensions that you want to make, get corresponding volume,

Take 0.75/1.0/1.5mm mini gel for example:

Add 2.0/2.7/4.0ml 2xResolving gel into the same volume of 2xResolving gel buffer, then add 40/60/80ul APS solution into this mixture, mix well.

 

Attention: 

Just shake it a few times by your hand, do not blow repeatedly with the pipette

It is not necessary to pour all the gel mixture liquid into the glass plate, and some should be left in the cup to observe the condensation of the gel mixture

 

 

3. Promptly pipette resolving gel mixture produced by Step 2 into the assembled gel plates evenly from side to side. Add a small layer of water or Ethanol in order to produce a clean, straight top of the Resolving Gel; allow Resolving Gel to dry (about 10 minutes). Then pour off water or Ethanol.

 

4. Prepare Stacking gel mixture.

According to gel dimensions that you want to make, get corresponding volume,

Take 0.75/1.0/1.5mm mini gel for example:

Add 0.5/0.75/1.0ml 2xStacking gel into the same volume of 2xStacking gel buffer, then add 10/15/20ul APS solution into this mixture, mix well.

 

Attention: 

Just shake it a few times by your hand, do not blow repeatedly with the pipette

It is not necessary to pour all the gel mixture liquid into the glass plate, and some should be left in the cup to observe the condensation of the gel mixture

 

5. Promptly pipette Stacking gel mixture produced by Step 4 into the assembled gel plates on top of the Resolving Gel, evenly from side to side.

 

6. Insert the comb, and prevent air bubbles from persisting. Allow Stacking gel to dry (~10min), use immediately, or store 4°C wrapped in plastic wrap.