SDS-PAGE Sample Loading Buffer (Reducing and Denaturing, 5X)

Overview

Tested applications

SDS-PAGE, Western blotting, Dot blotting

Product Picture

Properties

Form

Liquid

Storage instruction

Store at -20°C. 

Applications

Highlights

1. Ready-to-use: take and use at any time, without preparation
 

2. Reducing agent: TCEP has higher stability and better reduction effect
 

3. No unpleasant smell: reducing agent TCEP has no unpleasant smell, replacing traditional DTT or 2-ME

Protocols

1. Melt SDS-PAGE sample loading buffer (5X) in a water bath at room temperature or no more than 37°C in order to change it from solid into liquid, then take it out and store at room temperature immediately after melting in the water bath. Try to avoid putting it in the water bath for a long time. 

 

2. Mix protein sample and SDS-PAGE sample loading buffer (5X) according to the proportion of adding 1 μL protein sample loading buffer (5X) to every 4 μL protein sample. 

 

3. Heat at 100 ? or boiling water bath for 10 minutes to fully denature the protein. 

 

4. After cooling to room temperature, directly load the sample into the SDS-PAGE glue sampling hole. 

 

5. Electrophoresis usually stops when the blue dye reaches the bottom of the gel.