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    Anti-Shellfish beta Tubulin mouse mAb - Loading Control

    Catalog number :AT1612
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    The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments, and tubulins comprises the microtubule building block. Tubulin is a globular dimeric protein of alpha/beta chains and it has five distinct forms labeled as -a, -b,-g, -d and -e tubulin. a/b tubulins generates heterodimers which multimerize to form microtubule filaments. Several b tubulin isoforms such as b1, b2, b3, b4, b5, b6, b8 etc have been identified among which b1/b4 are present in cytosol and b2 is present in nuclei/nucleoplasm. d/e tubulins are associated with centrosomes, whereas the g tubulin forms the gammasome, which is required for nucleating microtubule filaments at centrosomes. Several cellular movements are mediated by microtubule action which includes the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, nerve-cell axon migration etc and these movements result from competitive microtubule polymerization/depolymerization or through the actions of microtubule motor proteins.
    Overview
    Reactivity
    Shellfish
    Tested applications
    WB (1:1000),
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user. 
    Specificity
    β-Tubulin Antibody detects endogenous levels of Shellfish β-Tubulin
    Properties
    Immunogen
    Recombinant full length protein, corresponding to amino acids 1-450 of Shellfish Tubulin
    Clonality
    Monoclonal, clone number: 10F2
    Isotype
    Mouse IgG1
    Form
    Liquid, 100 μg at 1mg/ml
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C 
    Applications
    WB Image

    Western blot analysis of 40ug of Shell fish tissue lysates at 1:1000 dilution using beta Tubulin antibody 
     
    Predicted band size: 55KDa 
    Observed band size: 55KDa

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