Anti-Shrimp beta Tubulin Mouse mAb
Catalog number :AT2200
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins.
- Overview
- Description
- anti- Shrimp beta Tubulin mouse monoclonal antibody - Loading Control
- Reactivity
- Shrimp
- Tested applications
- WB : 1/1000-1/10000. Predicted molecular weight: 55 kDa.Optimal dilutions/concentrations should be determined by the end user.
- Specificity
- This antibody detects endogenous levels of beta Tubulin of Shrimp.
- Properties
- Immunogen
- a synthetic peptide corresponding to the amino terminus of shrimp β-tubulin.
- Clonality
- Monoclonal, clone number: 6M4
- Isotype
- Mouse IgG1
- Form
- Mouse monoclonal antibody in 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 1 mg/mL (0.1% w/v) BSA (IgG free, protease free) as a stabilizer and 0.02% (w/v) sodium azide as preservative.
- Storage instruction
- Store at -20°C, Avoid freeze / thaw cycle. Do not aliquot the antibody.
- Host
- Mouse
- Applications
- WB Image
Western blot analysis of 40µg of tissue lysates at 1:2000 dilution using beta Tubulin antibody (Cat No.: AT2200)Predicted band size: 55KDa, Observed band size: 55KDaLane 1: shrimp gill tissue lysatesRecommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number:AT0098)Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)
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