The 5X All-In-One RT MasterMix for reverse transcription is efficient and fast for the synthesis of the first strand cDNA, and also includes the steps for removing genomic DNA.
During RNA extraction, contamination of genomic DNA (gDNA) is crucial for downstream experiments Impact, it can lead to false positives and incorrect judgments of gene expression levels. Therefore, efficiently removing gDNA is crucial for ensuring the success of experiments.
This RT MasterMix contains the synthesis of the first strand cDNA all reagents, inluding Oligo(dT) and Random Primers, except for RNA templates.
Poly (A)+mRNA and total RNA can be used for the synthesis of first strand cDNA.
The cDNA product (60°C incubation for 5 minutes) can be directly used for downstream qPCR reactions, and 60°C incubation for 10 minutes can be used for downstream PCR reactions.
60°C High-temperature Reverse Transcription is beneficial for the elimination of RNA secondary structure, the denaturation of RNA templates, and the synthesis of the first strand.
60°C High-temperature Reverse Transcription is beneficial for the elimination of RNA secondary structure, the denaturation of RNA templates, and the synthesis of the first strand.
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