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    Smart-Biotin™ RNA Biotin Labeling kit (T7 in vitro transcription)
    Catalog number IF9670
    DescriptionSmart-Biotin™ RNA Biotin Labeling kit (T7 in vitro transcription)
    Work Flow ImageT7-IVT
    Smart-ChIP™ ChIP Kit
    Catalog number ChIP-1001
    DescriptionChromatin Immunoprecipitation Assay kit, ChIP kit
    Highlights
    1. This reagent kit is suitable for adherent cells, suspended cells, animal tissues, and plant tissues. It has targeted and detailed steps to process these sample types, making it a true multi in one Kit.

    2. This kit contains all reagents for transcription factor class Cross linked ChIP (Ultrasound, Protein DNA Weak Binding) that require cross-linking treatment.

    3. This kit also includes all reagents for histone modified Native ChIP (MNase digestion, protein DNA strong binding) that do not require cross-linking treatment.

    4. This kit contains complete experimental procedures and data analysis plans for three downstream experiments of ChIP (PCR, qPCR, and NGS second-generation sequencing), and also includes all fluorescence quantitative PCR reagents in this ChIP Kit.

    5. This manual is a comprehensive technical manual for ChIP experiments, which thoroughly analyzes the key and difficult points in ChIP experiments, and provides targeted solutions to various key and difficult problems. It also includes the basic knowledge and context of epigenetics, summarizing detailed information on various types of histone modifications. After reading this nanny level manual, all difficulties will be easily solved, and ChIP is within your grasp.
    Smart-DNA-Pull™ DNA Pull-down Kit
    Catalog number IF9606
    DescriptionDNA-Protein Pull-down
    Highlights
    1. This kit is suitable for adherent cells, suspended cells, animal tissues, plant tissues, and purified recombinant proteins. It is suitable for various lengths and complexities of DNA probes labeled with terminal biotin. Proteins that are endogenous, overexpressed, or translated in vitro are suitable as sample types.

    2. This kit uses biotin labeled DNA probes to directly capture target proteins, such as transcription factors. No antibodies are required for pull down, and magnetic bead method is used without centrifugation. After completing the DNA labeling reaction, very little manual operation time is required (less than 3 hours).

    3. This reagent kit is suitable for the interaction between DNA sequences and DNA binding proteins such as transcription factors, transcription cofactors, DNA damage repair factors, etc. It can be used for downstream Western blot verification detection of known proteins and mass spectrometry identification of unknown proteins.

    4. This manual is a comprehensive technical manual for DNA Pull down experiments, summarizing detailed information on the interactions between DNA and various proteins. After reading this nanny level manual, all difficulties will be easily solved, and DNA Pull down is within your grasp.
    Smart-DRIP™ DRIP Kit
    Catalog number DRIP-1001
    DescriptionDNA-RNA hybrid Immunoprecipitation (DRIP) kit
    Highlights
    1. This kit is suitable for sample types containing R rings, such as bacteria, fungi, insects, adherent cells, suspended cells, animal tissues, and plant tissues.
     
    2. This kit is based on immunoprecipitation of S9.6 antibody and has high resolution, sensitivity, and specificity.
     
    3. This kit is suitable for two downstream experiments of DRIP (qPCR and NGS second-generation sequencing).
    Smart-EMSA™ EMSA Kit
    Catalog number IF9501
    DescriptionElectrophoretic Mobility Shift Assay kit, EMSA kit
    Highlights
    1. This kit is suitable for adherent cells, suspended cells, animal tissues, plant tissues, and purified recombinant proteins, and is a true multi in one kit.

    2. This kit contains reagents suitable for validation type EMSA, competitive type EMSA, and SuperShift hypermigration EMSA.

    3. This reagent kit uses chemiluminescence detection instead of radioactive isotope detection, with high sensitivity and no harm to the health of experimental personnel.

    4. This manual is a comprehensive technical manual for EMSA experiments, which thoroughly analyzes the key and difficult points in EMSA experiments, and provides targeted solutions to various key and difficult problems. It also includes the basic knowledge and context of epigenetics, summarizing detailed information on the interaction between DNA and various proteins. After reading this nanny level manual, all difficulties will be easily solved, and EMSA is in control.
    Smart-meRIP™ meRIP Kit
    Catalog number meRIP-1001
    Descriptionmethylated RNA Immunoprecipitation kit, meRIP kit
    Highlights
    This kit is suitable for methylation analysis of total RNA, mRNA, lncRNA, and circRNA from cells, animal tissues, plant tissues, blood, and embedded tissues.
     
    By combining different antibodies, different methylation types can be detected, such as m6A, m5C, and m7G.
     
    This kit is suitable for cytoplasmic RNA methylation and nuclear RNA methylation.
     
    This kit adopts an optimized elution system with a better signal-to-noise ratio than the MeRIP process using free m6A elution.
     
    This kit contains negative control IgG antibodies for easy quality control in IP experiments.
     
    This reagent kit is compatible with downstream RT qPCR and specific methylation of whole transcriptome sequencing.
     
    This Kit also includes RNA fragment column purification reagents for easy recovery of post IP RNA, without the need to purchase additional RNA purification kits.
    Smart-PCR™ PCR mastermix(2X)
    Catalog number AT15010
    DescriptionSmart-PCR™ PCR mastermix(2X) for standard PCR
    Smart-qPCR™ qPCR mastermix(2X)
    Catalog number AT15012
    DescriptionSmart-qPCR™ qPCR mastermix(2X)
    Smart-REMSA™ RNA EMSA Kit
    Catalog number IF9502
    DescriptionRNA Electrophoretic Mobility Shift Assay kit, REMSA kit
    Highlights
    1. This kit is suitable for adherent cells, suspended cells, animal tissues, plant tissues, and purified recombinant proteins, and is a true multi in one kit.

    2. This kit contains reagents suitable for validation RNA-EMSA, competitive RNA-EMSA, and SuperShift overtransition RNA-EMSA.

    3. This reagent kit uses chemiluminescence detection instead of radioactive isotope detection, with high sensitivity and no harm to the health of experimental personnel.

    4. This manual is a comprehensive technical manual for RNA EMSA experiments, which thoroughly analyzes the key and difficult points in REMSA experiments, and provides targeted solutions to various key and difficult problems. It also includes the basic knowledge and context of epigenetics, summarizing detailed information on the interaction between RNA and various proteins. After reading this nanny level manual, all difficulties will be easily solved, and RNA EMSA will be fully mastered.
    Smart-RIP™ RIP Kit
    Catalog number RIP-1001
    DescriptionRNA-RBP Immunoprecipitation kit, RIP kit
    Highlights
    1. This reagent kit is suitable for adherent cells, suspended cells, animal tissues, and plant tissues.

    2. This kit contains RIPs suitable for cytoplasmic RNA RBP interactions and nuclear RNA RBP interactions.

    3. This reagent kit is suitable for classic Native RIP and Crosslink cross-linking RIP (for particularly fragile RNA RBP interactions).

    4. This kit is suitable for downstream experiments (RT-PCR, RT qPCR, and transcriptome NGS second-generation sequencing).

    5. This manual is a comprehensive technical manual for RIP experiments. It also includes the basic knowledge and context of epigenetics, summarizing detailed information on the interaction between RNA and various proteins.
    Smart-RNA-Pull™ RNA Pull-down Kit
    Catalog number IF9608
    DescriptionRNA Protein Pull-down kit
    Highlights
    1. This kit is suitable for adherent cells, suspended cells, animal tissues, plant tissues, and purified recombinant proteins. It is suitable for various length and complexity RNA probes labeled with terminal biotin. Proteins that are endogenous, overexpressed, or translated in vitro are suitable as sample types.

    2. This kit uses biotin labeled RNA probes to directly capture the target protein. Pull down does not require antibodies or centrifugation. After completing the RNA labeling reaction, very little manual operation time is required (less than 3 hours).

    3. This reagent kit is suitable for the interaction between RBP and mRNA, RBP and LncRNA, RBP and miRNA, RBP and circRNA, as well as downstream Western blot verification detection of known proteins and mass spectrometry identification of unknown proteins.

    4. This manual is a comprehensive technical manual for RNA Pull down experiments, summarizing detailed information on the interaction between RNA and various proteins. After reading this nanny level manual, all difficulties will be easily solved, and RNA Pull down is within your grasp.
    Smart-RT™ RT mastermix(5X)
    Catalog number AT15011
    DescriptionThe 5X All-In-One RT MasterMix for reverse transcription(Add 4µL of mastermix for a 20µL total reaction system)
    Tn5 library prep PCR mastermix(2X)
    Catalog number AT15009
    DescriptionPCR mastermix(2X) for Tn5 library prep, No-Hot-Start, Strand Displacement
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