Anti-Strep-tag II Mouse mAb
Catalog number :AT0817
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II (WSHPQFEK).
- Overview
- Description
- Anti-Strep-tag II Mouse monoclonal antibody
- Tested applications
- WB : 1/1000-1/5000.Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
- Product Picture
- Specificity
- This antibody can recognize C-terminal, internal, and N-terminal Strep-tag II fusion proteins.
- Properties
- Immunogen
- A synthetic peptide WSHPQFEK coupled to KLH.
- Clonality
- 7D2
- Isotype
- Mouse IgG1
- Form
- Liquid, 1mg/mlPreservative: 0.01% Sodium AzideConstituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
- Storage instruction
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Host
- Mouse
- Applications
- WB Image
All lanes : Anti-Strep-tag II antibody at 1/5000 dilutionLane 1 : 293T whole cell lysateLane 2 : whole cell lysate of strep-tagged II protein expressing 293T cellsLysates/proteins at 30 µg per lane.12 % SDS-PAGE
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