This antibody detects a single clean band at 55kD representing beta Tubulin. This band is significantly reduced by using peptide blocking.
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins.
Anti-Fruit fly beta Tubulin Mouse mAb - Loading Control
Catalog number :AT1568
- Overview
- Description
- anti-β-Tubulin mouse mAb for Fruit fly
- Reactivity
- Reacts with: Fruit fly
- Tested applications
- WB(1:500-1:3000)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Properties
- Immunogen
- Recombinant full length protein of Fruit fly Tubulin
- Clonality
- 9B6
- Isotype
- Mouse IgG1
- Form
- Liquid, 100 μl at 1mg/ml
- Storage instruction
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C
- Applications
- WB Image
-
Western blot - beta Tubulin antibody- Loading Control (AT1568)
Western blot analysis of cell lysates(50ug) of Drosophila melanogaster at:1:1000 dilution using beta Tubulin antibody
Predicted band size: 55KDa
Observed band size: 55KDa
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