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    Anti-Fruit fly beta Tubulin Mouse mAb - Loading Control

    Catalog number :AT1568
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    This antibody detects a single clean band at 55kD representing beta Tubulin. This band is significantly reduced by using peptide blocking.
    The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins.
    Overview
    Description
    anti-β-Tubulin mouse mAb for Fruit fly
    Reactivity
    Reacts with:  Fruit fly
    Tested applications
    WB(1:500-1:3000)
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
    Properties
    Immunogen
    Recombinant full length protein of Fruit fly Tubulin
    Clonality
    9B6
    Isotype
    Mouse IgG1
    Form
    Liquid, 100 μl at 1mg/ml
    Storage instruction
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C
    Applications
    WB Image
    Western blot - beta Tubulin antibody- Loading Control (AT1568)
     

    Western blot analysis of cell lysates(50ug) of Drosophila melanogaster at:1:1000 dilution using beta Tubulin antibody

    Predicted band size: 55KDa
    Observed band size: 55KDa

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