• Traditional Co-IP and downstream WB and MS

• Protein-protein interaction Co-IP for downstream analysis in nonreducing conditions, such as enzyme activity assay, protein structure research

Liquid

Immunoprecipitation (IP),

Co-Immunoprecipitation (Co-IP)

• Traditional IP and downstream WB

• IP for downstream analysis in nonreducing conditions, such as enzyme activity assay, protein structure research

Kit Contents:

Sufficient For: 40 IP reactions, using 25 µL of magnetic beads slurry/reaction

 

• Protein A/G Magnetic Beads, 1 mL, Cat: IF0002, store at 4°C.

This beads concentration is 10 mg settled beads/ 1 mL beads slurry. > 600 µg of antibody (rabbit IgG or mouse IgG) can be bound per ml of beads slurry.

Note: Do not freeze, dry or centrifuge magnetic beads. This may cause irreversible aggregation and decreased binding capacity.

 

• Lysis Buffer for IP, 50 mL, Cat: IF6601, store at 4°C

• Wash Buffer for IP, 50 mL, Cat: IF9210, store at 4°C

• Elution Buffer, 4 mL, Cat: IF9213, store at 4°C

• Neutralization Buffer, 0.5 mL, Cat: IF9216, store at 4°C

• Sample Loading Buffer (Reducing and Denaturing, 5X), 1 mL, Cat: IF6740, store at -20°C

• Normal Rabbit IgG (Whole Molecule) Isotype Control,100 μL, Cat: AT1597, store at -20°C

• Normal Mouse IgG (Whole Molecule) Isotype Control,100 μL, Cat: AT1596, store at -20°C

Immunoprecipitation (IP),

Co-Immunoprecipitation (Co-IP),
Antibody purification

Isotype control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions.

An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

 

Immunoprecipitation (IP) and Co-Immunoprecipitation (Co-IP), Chromatin Immunoprecipitation (ChIP)

 

suitable for use at 25-40 µL resuspended agarose beads slurry per IP reaction. Optimal amount should be determined by the end user. 

1 mL of the product slurry is provided for 25-40 immunoprecipitation reactions

Kit Contents:

Sufficient For: 40 Co-IP reactions, using 25 µL of magnetic beads slurry/reaction

 

• Protein A/G Magnetic Beads, 1 mL, Cat: IF0002, store at 4°C. 

This beads concentration is 10 mg settled beads/ 1 mL beads slurry. > 600 µg of antibody (rabbit IgG or mouse IgG) can be bound per ml of beads slurry.

Note: Do not freeze, dry or centrifuge magnetic beads. This may cause irreversible aggregation and decreased binding capacity.

 

• Lysis Buffer for Co-IP, 24 mL, Cat: IF6603, store at 4°C
• Nuclear Lysis Buffer for Co-IP 24 mL, Cat: COIP001, store at 4°C

• Wash Buffer for Co-IP, 120 mL, Cat: IF9212, store at 4°C

• Elution Buffer for WB and MS, 2 mL, Cat: COIP002, store at 4°C
• Elution Buffer for non-denaturing, 4 mL, Cat: IF9213, store at 4°C

• Neutralization Buffer for non-denaturing, 0.5 mL, Cat: IF9216, store at 4°C
 

• Sample Loading Buffer (Reducing and Denaturing, 5X), 1 mL, Cat: IF6740, store at -20°C

• Normal Rabbit IgG (Whole Molecule) Isotype Control,100 μL, Cat: AT1597, store at -20°C

• Normal Mouse IgG (Whole Molecule) Isotype Control,100 μL, Cat: AT1596, store at -20°C
• DNase I (contains 10×DNase I Buffer), 100 U, Cat: COIP003, store at -20 °C

• QuickStain™ One-Step Protein Gel (PAGE) Blue Stain, 50mL, Cat: IF9217, store at room temperature