Dextrin Magnetic Beads for MBP Pull-Down Assay

Overview

Tested applications

MBP Pull-Down Assay. 

 

Optimal amount should be determined by the end user.

Specificity

Binding MBP-Tagged Fusion Protein.

Properties

Form

Magnetic Beads in Suspension (20% slurry), supplied in phosphate buffered saline (PBS) pH 7.4, with 0.02% sodium azide as preservatives.

Storage instruction

Store at 4°C. This product is stable for 12 months. Do not freeze, dry or centrifuge magnetic beads. This may cause irreversible aggregation and decreased binding capacity.

Bead Diameter: 40 µm

Concentration: 20% slurry

Binding Capacity: 50 to 70 mg MBP fusion protein per 1 mL settled beads (5mL 20% slurry). 

Applications

Protocols

Summary for MBP Pull-Down Assay

For detailed steps, please refer to the MBP pull-down kit used

 

I. Preparation of Bait Protein  

1: Bait from E. coli Expression Systems 

2: Bait from Insect Cell Expression Systems

3: Bait from Previously Purified Protein

 

II. Immobilization of Bait Protein 

A. Preparation of Dextrin Magnetic Beads

B. Immobilize Bait Protein to Dextrin Magnetic Beads using binding buffer

 

III. Preparation of Prey Protein

1: Prey Protein from E. coli or Baculovirus-Infected Insect Cell Lysate

2: Prey Protein from Mammalian Cell Lysate 

3: Prey from Previously Purified Protein

4: Prey Protein from In vitro Transcription/Translation Reaction

 

IV. Capture of Prey Protein 

 

V. Elution of Bait-Prey 

 

VI. SDS PAGE Gel Analysis or WB Analysis