ECL Femto-Detection™ Western Blotting Substrate

Overview

Description

ECL Femto-Detect™ Western Blotting Substrate

Tested applications

WB

Detection limit reaches fg grade protein

Product Picture

Properties

Components

Reagent A: 1 x 50ml; Reagent B: 1 x 50ml
Reagent A: 1 x 250ml; Reagent B: 1 x 250ml

Storage instruction

Store at +4°C

Applications

Highlights

High sensitivity (detect attomoles of protein) – ideal for detecting low abundance proteins (Detection limit reaches fg protein)

Quantitative – broad linear dynamic range of signal with respect to protein amount

Broad linear range (above 3 orders of magnitude) – detect low and high abundance proteins in the same exposure

Low background – high signal to noise ratio with film detection

Long lasting signal – carry out exposures at your convenience

Versatility – optimized for CCD imaging and compatible with film detection

Flexible - image chemiluminescence by CCD or film, image chemifluorescence with laser based fluorescence imaging systems

Work Flow Image

ECL chemiluminescence solution is used to detect antibodies that directly or indirectly label horseradish peroxidase (HRP) and its associated antigens. The detection principle is shown in Figure 1. After electrophoresis, the protein is transferred to the imprinted membrane, and the target protein on the membrane is bound by the primary antibody and the HRP labeled secondary antibody. After washing the film, incubate the film with ECL chemiluminescence solution at room temperature for several minutes, and the target protein can obtain clear bands on the X-ray film.

 

Engibody Company adopts innovative ECL chemiluminescence substrate to greatly improve detection sensitivity and prolong luminescence time. ECL chemiluminescence solution of our company is not only suitable for exposure imaging on X-ray film, but also suitable for CCD chemiluminescence imager imaging. It is easy to operate and is the preferred reagent for WB detection.

Application Figure 1

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Application Figure 3

Application Figure 4