ECL Pico-Detection™ Western Blotting Substrate

Overview

Description

Pico-Detect™  ECL Western Blotting Substrate

Tested applications

WB
Detection limit reaches pg grade protein

DETECTION PROCEDURE:

1. Mix Detection Reagents A & B at a 1:1 ratio.
2. Immediately add the mixture to the blot. Use 0.125 ml working solution per cm2 of membrane.
3. Incubate the blot for about 1 minute at room temperature.
4. Drain excess reagents.
5. Cover blot with a clear plastic sheet protector or clear plastic wrap.
6. Expose blot to X-ray film.

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Properties

Components

Reagent A: 1 x 50ml; Reagent B: 1 x 50ml
Reagent A: 1 x 250ml; Reagent B: 1 x 250ml

Applications

Highlights

Stronger signal than other chemiluminescent substrates for film imaging

Load less sample – conserve precious sample

Detect low pg amounts of protein – for detecting low abundance proteins

Use up to ten-times less antibody – conserve precious antibodies

Long lasting signal – carry out multiple exposures at your convenience without signal decay

Stable signal – carry out longer exposures to detect low abundance proteins

Work Flow Image

ECL chemiluminescence solution is used to detect antibodies that directly or indirectly label horseradish peroxidase (HRP) and its associated antigens. The detection principle is shown in Figure 1. After electrophoresis, the protein is transferred to the imprinted membrane, and the target protein on the membrane is bound by the primary antibody and the HRP labeled secondary antibody. After washing the film, incubate the film with ECL chemiluminescence solution at room temperature for several minutes, and the target protein can obtain clear bands on the X-ray film.

 

Engibody Company adopts innovative ECL chemiluminescence substrate to greatly improve detection sensitivity and prolong luminescence time. ECL chemiluminescence solution of our company is not only suitable for exposure imaging on X-ray film, but also suitable for CCD chemiluminescence imager imaging. It is easy to operate and is the preferred reagent for WB detection.

Application Figure 1

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