Anti-EGFP tag Mouse mAb

Overview

Description

anti-EGFP-Tag mouse monoclonal antibody

Reactivity

Any organism expressing EGFP.

Tested applications

WB : 1/1000-1/10000. 

IHC : 1/200-1/5000.

ICC/IF : 1/200-1/2000.

IP : 1/100-1/500.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Specificity

This antibody detects EGFP -tagged proteins exogenously expressed in cells or E. coli. This antibody does not detect RFP-tagged proteins. 

Please note that the EGFP tags add approximately 27 kDa to the molecular weight of the fusion protein.

The antibody recognizes the EGFP tag, which is fused to either the amino-terminus or carboxy-terminus of the target protein.

 

Properties

Immunogen

a Enhanced Green Fluorescent Protein (EGFP) recombinant protein.

Clonality

7K15

Isotype

Mouse IgG1

Form

Liquid
Mouse monoclonal antibody in 10 mM PBS, pH 7.4, 50% glycerol with 1 mg/mL (0.1% w/v) BSA (IgG free, protease free) as a stabilizer and 0.02% (w/v) sodium azide as preservative.

Storage instruction

store at -20°C long term. Avoid repeated freeze / thaw cycles.

Applications

WB Image

Western blot - anti-EGFP-Tag mouse mAb (AT0041)

 

All lanes: 

Lane: HEK293T cell lysate transfected with EGFP-Tagged protein, anti-EGFP-Tag mouse monoclonal antibody at 1/5000 dilution

 

Lysates/proteins at 25 µg per lane.

 

Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number: AT0098)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)