Goat Anti-Rabbit IgG(H+L) (iFluor 594)

Overview

Description

Goat-anti-rabbit IgG(H+L)(iFluor™ 594)

Reactivity

Rabbit

Tested applications

IF(ICC): 1/100 -1/2000

Product Picture

Properties

Clonality

Polyclonal

Form

Liquid

Storage instruction

Store at -20°C.and protected from prolonged exposure to light.

Conjugation

iFluor™ 594, Ex: 593nm, Em: 618nm.

Alternative to Texas Red, Alexa Fluor® 594 and DyLight™ 594

Applications

Application Image

Immunocytochemistry/ Immunofluorescence - Goat Anti- Rabbit IgG(H+L) (iFluor™ 594) (AT1553)

 

ICC/IF image of beta tubulin stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the beta Tubulin rabbit monoclonal antibody (AT0011) overnight at +4°C. The secondary antibody (red) was Goat-anti-Rabbit IgG(H+L)-iFluor™ 594 (AT1553, used at 1:500 for 1h). 

DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.