Goat Anti-Rabbit IgG(H+L) (iFluor 488)

Overview

Description

Goat-anti-rabbit IgG(H+L)(iFluor™ 488)

Reactivity

Rabbit

Tested applications

ICC/IF :1/100 - 1/2000.

Optimal dilutions/concentrations should be determined by the end user.

Product Picture

Properties

Clonality

Polyclonal

Form

Liquid

Storage instruction

Store at -20°C.and protected from prolonged exposure to light.

Conjugation

iFluor™ 488. Ex: 493nm, Em: 518nm

Applications

Application Image

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG(H+L) (iFluor™ 488)

ICC/IF image of beta Tubulin staining in HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (AT0011, Anti-beta Tubulin Rabbit mAb, 1:5000) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG(H+L) (iFluor™ 488) (AT1546, used at 1:2000 for 1h). DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.