Goat Anti-Mouse IgG(H+L) (iFluor 488)

Overview

Description

Goat-anti-mouse IgG(H+L)(iFluor™ 488)

Reactivity

mouse

Tested applications

ICC/IF: 1/100 - 1/2000,
Flow Cyt: The amount of the reagent is suggested to be used ≤ 1µg/106cells.

Product Picture

Properties

Clonality

Polyclonal

Form

Liquid

Storage instruction

Store at -20°C.and protected from prolonged exposure to light

Conjugation

iFluor™ 488. Ex: 493nm, Em: 518nm

Applications

ICC/IF Image

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin mouse monoclonal antibody(AT0007)

staining alpha Tubulin in MEF1 cells. The cells were fixed with 4% formaldehyde (10 minutes), permeabilized in 0.1% PBS-Triton X-100 for 5 minutes then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with AT0007 at 1/5000 dilution (shown in green) overnight at +4°C, then incubated with Goat Anti-Mouse IgG(H+L) (iFluor 488) (AT1547). Nuclear DNA was labeled in blue with DAPI.

 

This product gave a positive signal in 100% methanol (10 minutes) fixed MEF1 cells under the same testing conditions.

 

Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).