Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. P2710 quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 100µl to 0.5ml. Up to 500µg of antibody can be purified in each run.
The method involves capturing the antibody on the Protein L resin. Protein L has a high affinity for the Fc regions of IgG molecules from a variety of species. Once the antibody has bound to the Protein L, unwanted substances can be removed by simply washing the resin. Up to 500ug of antibody can be purified in each run.
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