The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
Anti-T7 tag Mouse mAb
Catalog number :AT0031
- Overview
- Description
- anti-T7-Tag mouse monoclonal antibody
- Reactivity
- this antibody detects a T7 fusion protein
- Tested applications
- WB : 1/1000-1/5000.Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
- Properties
- Immunogen
Synthetic peptide: MASMTGGQQMG-K, corresponding to amino acids 1-11 of Bacteriophage T7 gene10 (T7 tag®) conjugated to KLH.
- Clonality
- 4F5
- Isotype
- Mouse IgG1
- Form
Liquid
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
- Storage instruction
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Run BLAST with 
- Applications
- WB Image
Western Blot: T7 Epitope Tag Antibody(AT0031) - 40, 30, or 20 ug of HEK 293 whole cell lysate expressing a T7-tag fusion protein.
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