Anti-alpha Tubulin Rabbit mAb

Product

Anti-alpha Tubulin Rabbit mAb - Loading Control

Catalog number :AT0012

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.

Overview

Description: anti-alpha-Tubulin Rabbit monoclonal antibody

Reactivity: Mouse, Rat, Human

Tested applications: WB : 1/1000-1/5000. Predicted molecular weight: 50 kDa.

IHC : 1/50~1/200

ICC/IF : 1/50~1/200

IP : 1/50

FC : 1/50

Optimal dilutions/concentrations should be determined by the end user.

Properties

Immunogen: a synthetic peptide corresponding to the amino terminus of human alpha-tubulin.

Clonality: Monoclonal, clone number: 4A7

Isotype: Rabbit IgG

Form: Liquid
Rabbit monoclonal antibody in 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 1 mg/mL (0.1% w/v) BSA (IgG free, protease free) as a stabilizer and 0.02% (w/v) sodium azide as preservative.

Storage instruction: Store at -20°C, Avoid freeze / thaw cycle. Do not aliquot the antibody.

Applications

WB Image: Western blot - alpha Tubulin antibody - Loading Control (AT0012)

All lanes: Anti- alpha Tubulin antibody - Loading Control at 1/2000 dilution

(1) Hela cell lysate; (2) NIH/3T3 cell lysate.

Observed band size: 50KDa

Recommended secondary antibody: Goat anti rabbit IgG-HRP (Engibody, Catalog number:AT0097)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)