RNA pull-down Kit

Catalog number	circRNA-pull-1001
Description	Smart-circ-Pull™ circRNA Pull-down Kit (circRNA Protein Pull-down)
Highlights	1. This kit is suitable for adherent cells, suspension cells, animal tissues, and plant tissues, and can be used with endogenous, overexpressed, purified recombinant proteins, or proteins translated in vitro as sample types. It is applicable to biotin-labeled circular RNA probes of various lengths and complexities. 2. This kit directly captures the target protein using biotin-labeled circular RNA probes. Antibodies and centrifugation are not required during pull-down. After the RNA labeling reaction, only minimal manual operation time is needed (less than 3 hours). 3. This kit is suitable for detecting the interaction between RBP and circRNA, downstream validation via Western blot for known proteins, and mass spectrometry identification for unknown proteins. 4. This manual serves as a comprehensive technical guide for circRNA Pull-down experiments. After reading this step-by-step guide, all difficulties will be effortlessly resolved, and RNA Pull-down will be fully within your grasp.

Catalog number	miRNA-pull-1001
Description	Smart-mi-Pull™ miRNA Pull-down Kit (miRNA-Protein Pull-down)
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Catalog number	IF9608
Description	RNA Protein Pull-down kit
Highlights	This kit is suitable for adherent cells, suspended cells, animal tissues, plant tissues, and purified recombinant proteins. It is suitable for various length and complexity RNA probes labeled with terminal biotin. Proteins that are endogenous, overexpressed, or translated in vitro are suitable as sample types. This kit uses biotin labeled RNA probes to directly capture the target protein. Pull down does not require antibodies or centrifugation. After completing the RNA labeling reaction, very little manual operation time is required (less than 3 hours). This reagent kit is suitable for the interaction between RBP and mRNA, RBP and LncRNA, RBP and miRNA, RBP and circRNA, as well as downstream Western blot verification detection of known proteins and mass spectrometry identification of unknown proteins. This manual is a comprehensive technical manual for RNA Pull down experiments, summarizing detailed information on the interaction between RNA and various proteins. After reading this nanny level manual, all difficulties will be easily solved, and RNA Pull down is within your grasp.