QuickBlock™ 10-Minute Protein-Free Blocking Buffer

Overview

Description

QuickBlock™ 10 minute protein free blocking buffer, which is an efficient and fast blocking buffer. It is used in the blocking stage of Western Blot, and also in the dilution of antibodies (primary antibody and secondary antibody) in the Western Blot process. Pure chemical formula without protein can effectively prevent protein cross reaction and effectively improve the signal to noise ratio of the detected signal in a short time.

 

Furthermore, Protein-Free Blocking Buffer is compatible with antibodies and avidin/biotin based systems and results in high signal to background ratios.

Tested applications

Blocking of membrane (PVDF or NC) after transferring of proteins in western blot

Properties

Form

Liquid

Storage instruction

Store at 4°C

Applications

Highlights

1. Protein free blocking agent, no protein interference
 

2. Eliminate or minimize cross reactivity with animal source antibodies and antigens
 

3. High signal to background ratios
 

4. Ready-to-use
 

5. Suitable for fluorescent western blot and chemiluminescent western blot 
 

6. Suitable for detection of phosphorylated protein 
 

7. Ultra-rapid, 10 minutes
 

8. Antibody saving, diluted antibody can prolong the storage time, increase the number of antibody reuse, and greatly improve the utilization rate of antibody

Application Figure 1

1. Not only the operation time is short, but also the signal-to-noise ratio is effectively improved.

Application Figure 2

2. Pure chemical formula, no protein, diluted antibody can prolong the storage time, increase the number of antibody reuse, and greatly improve the utilization rate of antibody

Protocols

 

1. After the membrane transfer, wash the membrane with TBST.

 

2. According to the size of the membrane, pour a certain volume of QuickBlock™ into the container to ensure that the liquid can fully submerge the membrane.

 

3. Clamp one corner of the membrane with tweezers, place the membrane in QuickBlock™ solution, make the solution completely immerse the membrane, and place it on a horizontal shaking table at room temperature for about 10 minutes.

 

4. The blocked membrane can be used for subsequent experiments such as the incubation of the first antibody (the incubation effect is better if the antibody is diluted with QuickBlock™).

 

 

 

Attention:

 

1. It is not recommended to reuse this product, because with the increase of the number of reuses, the effective ingredients will be reduced due to the transfer to the membrane, and repeated use may lead to the failure to ensure the blocking effect. Please repeat the use of this product according to the facts within a reasonable range use.

 

2. When the product is received, foam may appear on the surface of the liquid (caused by shaking during transportation), which can be used normally after the foam dissipates.