QuickStain™ One-Step Protein Gel (PAGE) Blue Stain

Overview

Description

QuickStain™ has a major advantage over conventional Coomassie Blue staining as it eliminates the lengthy fixation step. A further time saving factor of QuickStain™ is that no mixing or preparation is required, simply pour QuickStain™ onto gels and protein bands develops within 5-10 minutes. Complete staining achieved in 1 hour. QuickStain™ only stains proteins, leaving a crystal clear background producing high band visibility.

Product Picture

Properties

Form

Liquid

Storage instruction

Store at RT. 
It is stored at room temperature and is valid for one year after opening. If stored at 4°C, crystals will form, and can be used again after melting at 37°C.

Applications

Application Image

Highlights

1. Ultrafast dyeing - color can be developed in 5min;

2. One step method - no need for excessive washing, fixation, microwave heating and decolorization;

3. High sensitivity - 10ng protein band can be detected;

4. Clear background - high signal/noise ratio;

5. Flexible - no excessive coloring;

6. Reliable - batch consistency;

7. Economical - only 25ml dye solution is needed for each glue;

8. Safe ingredients - non-toxic, no need for fume hood or solvent treatment;

9. No methanol - no gel shrinkage or protein methylation;

10. No acetic acid - no protein acetylation;

11. Mass Spectrometry Compatibility - No residual methylation or acetylation.

Work Flow Figure 1

Work Flow Figure 2

Application Figure 1

Protocols

Must read before use:

 

1 If a little sediment is found in the bottle before use, please mix well before use.

 

2 The following "method of use" is for 0.75mm thickness, 10 × 10 cm gel staining procedure.

 

Usage Procedure:

 

1. Remove the PAGE gel after electrophoresis and put it into a plastic container, and rinse it with distilled water.

 

2. Discard distilled water, add an appropriate amount of dye solution (if the glue surface has just been covered), shake it on the shaking table at room temperature, and determine the dyeing time according to the following table.

 

Amount of protein to be tested	Dyeing time
>1 μ g	2 minutes
100ng-1 μ g	10 minutes
10ng-100ng60	60 minutes

 

3. Observe and record the results.

 

 

Attachment: Mass spectrum processing procedure

 

1. Cut the protein strip to be tested and place it in a 1.5ml centrifuge tube.

 

2. Add 1ml of 30% ethanol or 30% acetone (30% acetic acid can also be used, but it will cause N-terminal acylation of protein).

 

3. Handle for 30 minutes.

 

4. Repeat steps 2 and 3 until all dyes are removed. Generally, dye can be removed after 2-3 times of treatment.

 

5. Mass spectrum analysis.

 

Precautions:

 

1. Various parameters in the use method are only for the area 8 × 10cm or 10 × 10cm, 0.75mm thick gel. If larger or thicker gel is used, please add more dye solution and extend the dyeing time.

 

2. The rinsing, fixation and decolorization steps required by conventional dyeing are unnecessary.

 

3. This dye solution can be reused for 1-2 times, and the sensitivity will decrease slightly. Please extend the dyeing time.

 

4. This dye solution is slightly corrosive, please wear gloves. After use, please cover the bottle and seal it at room temperature.

Troubleshooting Guide

Question	Answer
QuickStain ™ What is the best temperature for dyeing glue?	To achieve the best dyeing effect, room temperature (20-25°C) dyeing is recommended.
QuickStain ™ Does the solution need to be stored at low temperature?	Low temperature storage is not required, and normal temperature storage (20-25 ?) is enough. FastBlue will be crystallized when stored at 4-8°C, and can be used again after melting at 37°C.
QuickStain ™ What is the dyeing principle of?	The dyeing principle is the same as that of the classical Coomassie Brilliant Blue G250 (combined with basic amino acids in proteins), with the advantage of being faster and more sensitive.
QuickStain ™ Can gel be preserved in distilled water after dyeing?	Yes, distilled water is the simplest to store, and it can also be stored in diluted glacial acetic acid.
QuickStain ™ Can the dyed gel be analyzed by mass spectrometry?	sure. Refer to "Mass Spectrum Processing Procedure"
QuickStain ™ Can dyed gel be used for Weatern Blot?	may not. Protein staining is irreversible, and stained gel cannot be used as downstream membrane transfer operation.
QuickStain ™ Can I reuse it?	It can be reused for 2-3 times, but the sensitivity has decreased, which can be remedied by prolonging the dyeing time.
QuickStain ™ Why does the band appear smear like tail after gel overnight staining?	Staining overnight will not over stain the gel. The reason for smear like tailing is that the sample loading is too large, because FastBlue has high dyeing sensitivity. In addition, proteins with small molecular weight will diffuse during overnight dyeing. We recommend that the dyeing be completed within 60 minutes.
QuickStain ™ Does it fix gel?	It is a aqueous solution and has no function of fixing gel. However, since the staining will be completed within 60 minutes, it is not necessary to fix the gel before staining.