Anti-STAT3(phospho S727) Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Product

Anti-STAT3(phospho S727) Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

Catalog number :AT0605

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is required for murine fetal development. Research studies have shown that Stat3 is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding. Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways. Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage. It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain.

Overview

Reactivity: Mouse, Rat, Human

Tested applications: Western Blot: 1:1000

Immunohistochemistry: 1:500

ICC/IF: 1:250

ChIP, CUT&RUN and CUT&Tag

Specificity: Phospho-Stat3 (Ser727) Antibody recognizes endogenous levels of Stat3 only when phosphorylated at Ser727. The antibody does not significantly cross-react with the corresponding phospho-serines of other Stat proteins.

Properties

Immunogen: Synthetic peptide corresponding to Human STAT3 aa 700 to the C-terminus (phospho S727) conjugated to Keyhole Limpet Haemocyanin (KLH).

Database link: P40763

Clonality: Monoclonal, clone number: 5ER4

Isotype: Rabbit IgG

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C

Applications

WB Image: Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL 6 for 15 minutes. PVDF membrane was probed with 1 µg/mL Anti-Phospho-STAT3 (S727) Rabbit Polyclonal Antibody (Catalog: AT0605) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog: AT0097). A specific band for Phospho STAT3 (S727) was detected at approximately 110 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Anti STAT3 Antibody (lower panel, Catalog: AT0358).

Anti-STAT3(phospho S727) Rabbit mAb - ChIP, CUT&RUN and CUT&Tag Grade

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